您好！歡迎訪問上海起發(fā)實驗試劑有限公司網(wǎng)站！
全國服務咨詢熱線:
15921799099
15921799099
Bacteria Resistance：KanamycinMammalian Selection：-
載體特點：與pGADT7-T構成Matchmaker Two-Hybrid System 3的雙雜交正對照。
pGBKT7-53 encodes a fusion of the murine p53 protein （a.a. 72–390） and the GAL4 DNA-BD （a.a. 1–147）. The murine p53 cDNA （GenBank Accession #K01700） was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. （1993）; plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.
Bacteria Resistance：KanamycinMammalian Selection：-
載體特點：Matchmaker Two-Hybrid System 3的負對照。
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein （a.a. 66–230） and the GAL4 DNA-BD （a.a. 1–147）. The lamin C cDNA insert （GenBank Accession #M13451） was derived from the plasmid referenced in Bar et al. （1993a）. Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
地址：上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
郵箱：xs1@78bio.com
傳真：021-50724961
電話
QQ咨詢
4006551678
當前位置：