成人18视频免费69_аⅴ中文天堂最新版在线_亚洲天堂在线视频播放_99色免费视频_91国自产精品中文字幕亚洲_午夜a级理论片在线播放琪琪

您好!歡迎訪問上海起發(fā)實(shí)驗(yàn)試劑有限公司網(wǎng)站!
全國服務(wù)咨詢熱線:

15921799099

當(dāng)前位置:首頁 > 產(chǎn)品中心 > 自產(chǎn)產(chǎn)品 > 試劑 > Phosphosolutions公司Anti-Adenylate Cyclase III-NEW產(chǎn)品代理

Phosphosolutions公司Anti-Adenylate Cyclase III-NEW產(chǎn)品代理

簡要描述:公司概況

背景
基因工程-- Phosphosolutions是*代可以完整描繪人體的遺傳物質(zhì)序列的企業(yè)。
蛋白質(zhì)體學(xué)項(xiàng)目:Phosphosolutions是第二代試圖將所有體內(nèi)蛋白質(zhì)表達(dá)出來的企業(yè)。
PhosphoSolutions公司—第三步我們將超越蛋白質(zhì)體學(xué) 進(jìn)而 專注于磷蛋白質(zhì)。

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):經(jīng)銷商
  • 更新時(shí)間:2025-06-01
  • 訪  問  量:2344

詳細(xì)介紹

 our focus 專業(yè)特色

PhosphoSolutions公司專注于蛋白質(zhì)組學(xué)中的一個(gè)(10-20%)含量的小部分磷蛋白質(zhì)。磷蛋白是監(jiān)管控制組蛋白質(zhì)的關(guān)鍵,這一部分是被稱為phosphosome蛋白質(zhì)。磷蛋白被認(rèn)為是在神經(jīng)系統(tǒng)疾病如老年癡呆癥和癌癥方面的關(guān)鍵元素,實(shí)質(zhì)上,phosphosome是蛋白質(zhì)組學(xué)作物的精華。

 

公司目標(biāo)

簡明概述:我們要成為世界上的磷蛋白組的提供者。

方案#1, 特異性磷抗體:首先我們要準(zhǔn)備磷蛋白組。在激活或磷酸化狀態(tài)下磷蛋白組是蛋白質(zhì)識(shí)別研究中的*關(guān)鍵工具。

 

Antibodies 抗體

特異性磷抗體:Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations (Nestler and Greengard, 1984). Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies (Lerner, 1982;Sutcliffe et al., 1983), an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.

The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin (KLH) (Ross et al., 1981). Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase (Nairn et al., 1982). A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.

Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site(s) of phosphorylation (Czernik et al., 1991)). In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation (Czernik et al., 1991). Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art (Czernik et al., 1995;Czernik et al., 1996). Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.

參考文獻(xiàn)

1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P (1991) Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.

2. Czernik AJ, Mathers J, Mische SM (1997) Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.

3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM (1995) Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.

4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.

5. Nairn AC, Detre JA, Casnellie JE, Greengard P (1982) Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature (Lond ) 299: 734-736.

6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 

8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA (1983) Antibodies that react with predetermined sites on proteins. Science 219: 660-666.

主營產(chǎn)品清單如下:

Item: Anti-Adenylate Cyclase III-NEW!
Category:  
Sub-Category:  
SKU/Catalog Number: 85-AC3
Datasheet:  click to view
SKU Price Formulation Applications Amount Qty
85-AC3 $325.00 affinity purified polyclonal antibody WB, IF 100 ul

 

產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
上海起發(fā)實(shí)驗(yàn)試劑有限公司
地址:上海浦東川沙鎮(zhèn)川沙路6619號(hào)上海起發(fā)實(shí)驗(yàn)試劑有限公司
郵箱:xs1@78bio.com
傳真:021-50724961
關(guān)注我們
歡迎您關(guān)注我們的微信公眾號(hào)了解更多信息:
歡迎您關(guān)注我們的微信公眾號(hào)
了解更多信息
主站蜘蛛池模板: 中国av视频| 日韩视频一区在线 | 欧美老妇60 | 榴莲视频黄色软件 | 中字幕av | 欧美特黄三级成人 | www.youjizz.com日韩 | 自拍偷区亚洲综合美利坚 | 国产成人精品福利色多多 | 天堂在线www中文 | 精产国品一区二区三区四区 | 国产情侣av自拍 | 91精品一久久香蕉国产线看观看软件 | 天天操夜夜操很很操 | 26uuu狠狠色| 少妇丰满爆乳被呻吟进入 | 一级特黄少妇高清毛片 | 亚洲国产一二区 | 亚洲成人av动漫 | 久久久SS麻豆欧美国产日韩 | 悠悠色在线 | 免费大片观看视频网站 | 青青青在线视频播放 | 中文字幕日日夜夜 | 给我播放片在线观看8集免费 | 亚洲 高清 成人 动漫 | 高清毛片免费 | 欧美精品日韩少妇 | 老人与老人a级毛片视频 | 午夜精品视频在线 | 在线免费看黄的网站 | 色阁五月天 | 国产伦精品一区二区三区视频无 | 国产精品网站在线看 | 久久老子午夜精品无码 | 91社区福利 | 日韩在线播 | 日本欧美一区二区免费不卡 | 美女脱内衣内裤视频 | 欧美真人性做爰一二区 | 超碰在线人人艹 |